{"id":1117,"date":"2018-08-14T03:50:14","date_gmt":"2018-08-14T03:50:14","guid":{"rendered":"https:\/\/integrativewellnessandmovement.com\/iwmbasicscience\/?p=1117"},"modified":"2018-08-14T03:50:14","modified_gmt":"2018-08-14T03:50:14","slug":"microbiology-basic-media-medium-for-growing-cultures","status":"publish","type":"post","link":"https:\/\/integrativewellnessandmovement.com\/iwmbasicscience\/microiology\/microbiology-basic-media-medium-for-growing-cultures\/","title":{"rendered":"Microbiology: Basic media\/medium for growing cultures."},"content":{"rendered":"<p>In order to grow organisms, you need to provide an adequate environment suitable for their growth.<\/p>\n<p>Things to consider are (but not limited to):<\/p>\n<ul>\n<li>Temperature. Does it grow best at room temperature or in an incubator?<\/li>\n<li>Aerobic, anaerobic, and everything inbetween.<\/li>\n<li>Acidity.<\/li>\n<li>Room to grow. You need to provide enough space for your organism to grow.<\/li>\n<li>Nutrients.<\/li>\n<\/ul>\n<p>Consider your <strong>culture medium<\/strong>\u2014basic building block nutrients for your organism to grow.<\/p>\n<p>A <strong>broth medium<\/strong> is a liquid nutrient environment. It is good for growing a large number of organisms in a limited volume (generally a test tube size). A loop is usually sufficient to inoculate the broth. Visually (naked eye) examine the sterile broth. Note it&#8217;s opacity and viscosity. After inoculation (and after adequate growth time), note the broth&#8217;s opacity. You should be able to notice a difference\u2014usually a &#8220;clouded&#8221; effect; particulates; maybe color change at the surface or bottom of a tube; and opacity.<\/p>\n<p>An <strong>agar medium<\/strong> is a semisolid (.ess than 1% agar) to solid medium (1.5-1.8% agar) suitable for a slant test tube, deep test tube, or plate. Agar comes from seaweed and contains galactose (no real nutritional value). One can mix in different nutrients (whatever you need for a task) into the agar base. Agar is liquid at 100 \u00b0C and solidifies at 40 \u00b0C.<\/p>\n<p>Reference<\/p>\n<p>Cappuccino,\u00a0J.\u00a0G., &amp; Welsh,\u00a0C. (2018).\u00a0<em>Microbiology: A laboratory manual<\/em>.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>In order to grow organisms, you need to provide an adequate environment suitable for their growth. Things to consider are (but not limited to): Temperature. Does it grow best at room temperature or in an incubator? Aerobic, anaerobic, and everything inbetween. Acidity. Room to grow. You need to provide enough space for your organism to [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[36],"tags":[],"class_list":["post-1117","post","type-post","status-publish","format-standard","hentry","category-microiology"],"_links":{"self":[{"href":"https:\/\/integrativewellnessandmovement.com\/iwmbasicscience\/wp-json\/wp\/v2\/posts\/1117","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/integrativewellnessandmovement.com\/iwmbasicscience\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/integrativewellnessandmovement.com\/iwmbasicscience\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/integrativewellnessandmovement.com\/iwmbasicscience\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/integrativewellnessandmovement.com\/iwmbasicscience\/wp-json\/wp\/v2\/comments?post=1117"}],"version-history":[{"count":2,"href":"https:\/\/integrativewellnessandmovement.com\/iwmbasicscience\/wp-json\/wp\/v2\/posts\/1117\/revisions"}],"predecessor-version":[{"id":1119,"href":"https:\/\/integrativewellnessandmovement.com\/iwmbasicscience\/wp-json\/wp\/v2\/posts\/1117\/revisions\/1119"}],"wp:attachment":[{"href":"https:\/\/integrativewellnessandmovement.com\/iwmbasicscience\/wp-json\/wp\/v2\/media?parent=1117"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/integrativewellnessandmovement.com\/iwmbasicscience\/wp-json\/wp\/v2\/categories?post=1117"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/integrativewellnessandmovement.com\/iwmbasicscience\/wp-json\/wp\/v2\/tags?post=1117"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}