{"id":1121,"date":"2018-08-14T01:55:19","date_gmt":"2018-08-14T01:55:19","guid":{"rendered":"https:\/\/integrativewellnessandmovement.com\/iwmbasicscience\/?p=1121"},"modified":"2018-08-14T03:03:59","modified_gmt":"2018-08-14T03:03:59","slug":"microbiology-streakplates","status":"publish","type":"post","link":"https:\/\/integrativewellnessandmovement.com\/iwmbasicscience\/microiology\/microbiology-streakplates\/","title":{"rendered":"Microbiology: Streak plates."},"content":{"rendered":"

Inoculations<\/strong><\/p>\n

Streak plate <\/strong>(Cappuccino & Welsh, 2017). Refer to Figure 12. Using aseptic technique:<\/p>\n

    \n
  1. Using a Sharpie marker, divide the plate into 4 quadrants labeled 1-4 clockwise.<\/li>\n
  2. Flame transfer loop and cool. Place a loopful of broth culture in quadrant-1 by dragging the loop across the periphery (close to the edge of plate) in a tight
    \nzigzag pattern.<\/li>\n
  3. Flame loop and cool. Turn plate 90\u00b0 Touch the loop at one corner of quadrant-1 and drag across to quadrant-2 in a similarly tight zigzag pattern.<\/li>\n
  4. Flame loop and cool. Turn plate 90\u00b0 Touch the loop at one corner of quadrant-2 and drag across to quadrant-3 in a similarly tight zigzag pattern.<\/li>\n
  5. Flame loop and cool. Turn plate 90\u00b0 Touch the loop at one corner of quadrant-3 and drag across to quadrant-4 and up through the middle\/center of the plate, carefully avoiding previously inoculated areas in quadrants 1-3.<\/li>\n
  6. Remember to store plates upside down<\/strong>: tape the lid to the plate; store it with the lid on the bottom. This allows for any condensation to drip onto the lid instead of on the medium\/culture itself.<\/li>\n<\/ol>\n

    \"\"<\/a><\/p>\n

    Figure 12<\/em>. Streak plate technique<\/p>\n

    \"\"<\/a><\/p>\n

    \"\"<\/a><\/p>\n

    Reference<\/p>\n

    Cappuccino,\u00a0J.\u00a0G., & Welsh,\u00a0C. (2018).\u00a0Microbiology: A laboratory manual<\/em>.<\/p>\n","protected":false},"excerpt":{"rendered":"

    Inoculations Streak plate (Cappuccino & Welsh, 2017). Refer to Figure 12. Using aseptic technique: Using a Sharpie marker, divide the plate into 4 quadrants labeled 1-4 clockwise. Flame transfer loop and cool. Place a loopful of broth culture in quadrant-1 by dragging the loop across the periphery (close to the edge of plate) in a […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[36],"tags":[],"class_list":["post-1121","post","type-post","status-publish","format-standard","hentry","category-microiology"],"_links":{"self":[{"href":"https:\/\/integrativewellnessandmovement.com\/iwmbasicscience\/wp-json\/wp\/v2\/posts\/1121","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/integrativewellnessandmovement.com\/iwmbasicscience\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/integrativewellnessandmovement.com\/iwmbasicscience\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/integrativewellnessandmovement.com\/iwmbasicscience\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/integrativewellnessandmovement.com\/iwmbasicscience\/wp-json\/wp\/v2\/comments?post=1121"}],"version-history":[{"count":3,"href":"https:\/\/integrativewellnessandmovement.com\/iwmbasicscience\/wp-json\/wp\/v2\/posts\/1121\/revisions"}],"predecessor-version":[{"id":1161,"href":"https:\/\/integrativewellnessandmovement.com\/iwmbasicscience\/wp-json\/wp\/v2\/posts\/1121\/revisions\/1161"}],"wp:attachment":[{"href":"https:\/\/integrativewellnessandmovement.com\/iwmbasicscience\/wp-json\/wp\/v2\/media?parent=1121"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/integrativewellnessandmovement.com\/iwmbasicscience\/wp-json\/wp\/v2\/categories?post=1121"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/integrativewellnessandmovement.com\/iwmbasicscience\/wp-json\/wp\/v2\/tags?post=1121"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}