{"id":1124,"date":"2018-08-14T01:56:35","date_gmt":"2018-08-14T01:56:35","guid":{"rendered":"https:\/\/integrativewellnessandmovement.com\/iwmbasicscience\/?p=1124"},"modified":"2018-08-14T03:09:19","modified_gmt":"2018-08-14T03:09:19","slug":"microbiology-slanttube","status":"publish","type":"post","link":"https:\/\/integrativewellnessandmovement.com\/iwmbasicscience\/microiology\/microbiology-slanttube\/","title":{"rendered":"Microbiology: Slant tube."},"content":{"rendered":"<p><strong>Inoculations<\/strong><\/p>\n<p><strong>Agar slant <\/strong>(Cappuccino &amp; Welsh, 2017). Using aseptic technique:<\/p>\n<ol>\n<li>Flame loop. Flame opening of broth culture.<\/li>\n<li>Dip loop into broth culture. Flame opening of broth culture.<\/li>\n<li>Open the sterile slant. Flame opening of the sterile slant.<\/li>\n<li>Using the loop (which has been dipped into the broth culture), drag loop across the agar slant in a zig-zag pattern from the end (bottom) up to the opening.<\/li>\n<li>Flame opening of slant tube and replace the cap. Flame loop.<\/li>\n<\/ol>\n<p><a href=\"https:\/\/integrativewellnessandmovement.com\/iwmbasicscience\/wp-content\/uploads\/2018\/08\/SlantExampleCfreundii.jpg\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-1162\" src=\"https:\/\/integrativewellnessandmovement.com\/iwmbasicscience\/wp-content\/uploads\/2018\/08\/SlantExampleCfreundii.jpg\" alt=\"\" width=\"576\" height=\"1024\" srcset=\"https:\/\/integrativewellnessandmovement.com\/iwmbasicscience\/wp-content\/uploads\/2018\/08\/SlantExampleCfreundii.jpg 576w, https:\/\/integrativewellnessandmovement.com\/iwmbasicscience\/wp-content\/uploads\/2018\/08\/SlantExampleCfreundii-169x300.jpg 169w\" sizes=\"auto, (max-width: 576px) 100vw, 576px\" \/><\/a><\/p>\n<p><a href=\"https:\/\/integrativewellnessandmovement.com\/iwmbasicscience\/wp-content\/uploads\/2018\/08\/SlantExampleCfreundii0525more.jpg\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-1163\" src=\"https:\/\/integrativewellnessandmovement.com\/iwmbasicscience\/wp-content\/uploads\/2018\/08\/SlantExampleCfreundii0525more.jpg\" alt=\"\" width=\"576\" height=\"1024\" srcset=\"https:\/\/integrativewellnessandmovement.com\/iwmbasicscience\/wp-content\/uploads\/2018\/08\/SlantExampleCfreundii0525more.jpg 576w, https:\/\/integrativewellnessandmovement.com\/iwmbasicscience\/wp-content\/uploads\/2018\/08\/SlantExampleCfreundii0525more-169x300.jpg 169w\" sizes=\"auto, (max-width: 576px) 100vw, 576px\" \/><\/a><\/p>\n<p>Reference<\/p>\n<p>Cappuccino,\u00a0J.\u00a0G., &amp; Welsh,\u00a0C. (2018).\u00a0<em>Microbiology: A laboratory manual<\/em>.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Inoculations Agar slant (Cappuccino &amp; Welsh, 2017). Using aseptic technique: Flame loop. Flame opening of broth culture. Dip loop into broth culture. Flame opening of broth culture. Open the sterile slant. Flame opening of the sterile slant. Using the loop (which has been dipped into the broth culture), drag loop across the agar slant in [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[36],"tags":[],"class_list":["post-1124","post","type-post","status-publish","format-standard","hentry","category-microiology"],"_links":{"self":[{"href":"https:\/\/integrativewellnessandmovement.com\/iwmbasicscience\/wp-json\/wp\/v2\/posts\/1124","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/integrativewellnessandmovement.com\/iwmbasicscience\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/integrativewellnessandmovement.com\/iwmbasicscience\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/integrativewellnessandmovement.com\/iwmbasicscience\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/integrativewellnessandmovement.com\/iwmbasicscience\/wp-json\/wp\/v2\/comments?post=1124"}],"version-history":[{"count":2,"href":"https:\/\/integrativewellnessandmovement.com\/iwmbasicscience\/wp-json\/wp\/v2\/posts\/1124\/revisions"}],"predecessor-version":[{"id":1164,"href":"https:\/\/integrativewellnessandmovement.com\/iwmbasicscience\/wp-json\/wp\/v2\/posts\/1124\/revisions\/1164"}],"wp:attachment":[{"href":"https:\/\/integrativewellnessandmovement.com\/iwmbasicscience\/wp-json\/wp\/v2\/media?parent=1124"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/integrativewellnessandmovement.com\/iwmbasicscience\/wp-json\/wp\/v2\/categories?post=1124"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/integrativewellnessandmovement.com\/iwmbasicscience\/wp-json\/wp\/v2\/tags?post=1124"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}