{"id":1133,"date":"2018-08-14T02:14:14","date_gmt":"2018-08-14T02:14:14","guid":{"rendered":"https:\/\/integrativewellnessandmovement.com\/iwmbasicscience\/?p=1133"},"modified":"2018-08-14T02:14:40","modified_gmt":"2018-08-14T02:14:40","slug":"microbiology-macconkey","status":"publish","type":"post","link":"https:\/\/integrativewellnessandmovement.com\/iwmbasicscience\/microiology\/microbiology-macconkey\/","title":{"rendered":"Microbiology: MacConkey agar."},"content":{"rendered":"<p><strong>MacConkey Agar <\/strong>(Cappuccino &amp; Welsh, 2017).<\/p>\n<p><strong>Description<\/strong>. MacConkey agar is selective and differential medium. It is selective for gram-negative bacteria as crystal violet (in MacConkey) inhibits gram-positive organisms. Lactose, bile salts, and pH indicator neutral red in MacConkey agar allow differentiation between coliform bacilli (lactose fermentation produces acidic end-products indicated by red-colored growth and pink medium surrounding growth) and dysentery\/typhoid\/paratyphoid bacilli (non-lactose fermenters, no acidic end-products, appear tan\/clear).<\/p>\n<p><strong>Clinical significance<\/strong>. MacConkey can help differentiate between gram-negative and gram-positive bacteria when trying to culture a pathogen from a wound sample.<\/p>\n<p><strong>Summary of main steps<\/strong>. It is assumed that the reader knows aseptic technique and basic inoculation.<\/p>\n<ol>\n<li>Using aseptic technique, inoculate a MacConkey agar plate.<\/li>\n<li>Incubate inverted plate (lid on bottom) at 37\u00b0C for 24-48 hours.<\/li>\n<li>Observe growth.<\/li>\n<\/ol>\n<p><a href=\"https:\/\/integrativewellnessandmovement.com\/iwmbasicscience\/wp-content\/uploads\/2018\/08\/0531Mac2SM.jpg\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-1134\" src=\"https:\/\/integrativewellnessandmovement.com\/iwmbasicscience\/wp-content\/uploads\/2018\/08\/0531Mac2SM.jpg\" alt=\"\" width=\"720\" height=\"975\" srcset=\"https:\/\/integrativewellnessandmovement.com\/iwmbasicscience\/wp-content\/uploads\/2018\/08\/0531Mac2SM.jpg 720w, https:\/\/integrativewellnessandmovement.com\/iwmbasicscience\/wp-content\/uploads\/2018\/08\/0531Mac2SM-222x300.jpg 222w\" sizes=\"auto, (max-width: 720px) 100vw, 720px\" \/><\/a><\/p>\n<p><a href=\"https:\/\/integrativewellnessandmovement.com\/iwmbasicscience\/wp-content\/uploads\/2018\/08\/0531MAc3SM.jpg\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-1135\" src=\"https:\/\/integrativewellnessandmovement.com\/iwmbasicscience\/wp-content\/uploads\/2018\/08\/0531MAc3SM.jpg\" alt=\"\" width=\"720\" height=\"1160\" srcset=\"https:\/\/integrativewellnessandmovement.com\/iwmbasicscience\/wp-content\/uploads\/2018\/08\/0531MAc3SM.jpg 720w, https:\/\/integrativewellnessandmovement.com\/iwmbasicscience\/wp-content\/uploads\/2018\/08\/0531MAc3SM-186x300.jpg 186w, https:\/\/integrativewellnessandmovement.com\/iwmbasicscience\/wp-content\/uploads\/2018\/08\/0531MAc3SM-636x1024.jpg 636w\" sizes=\"auto, (max-width: 720px) 100vw, 720px\" \/><\/a><\/p>\n<p><a href=\"https:\/\/integrativewellnessandmovement.com\/iwmbasicscience\/wp-content\/uploads\/2018\/08\/0531Mac4SM.jpg\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-1136\" src=\"https:\/\/integrativewellnessandmovement.com\/iwmbasicscience\/wp-content\/uploads\/2018\/08\/0531Mac4SM.jpg\" alt=\"\" width=\"720\" height=\"1122\" srcset=\"https:\/\/integrativewellnessandmovement.com\/iwmbasicscience\/wp-content\/uploads\/2018\/08\/0531Mac4SM.jpg 720w, https:\/\/integrativewellnessandmovement.com\/iwmbasicscience\/wp-content\/uploads\/2018\/08\/0531Mac4SM-193x300.jpg 193w, https:\/\/integrativewellnessandmovement.com\/iwmbasicscience\/wp-content\/uploads\/2018\/08\/0531Mac4SM-657x1024.jpg 657w\" sizes=\"auto, (max-width: 720px) 100vw, 720px\" \/><\/a><\/p>\n<p>Reference<\/p>\n<p>Cappuccino,\u00a0J.\u00a0G., &amp; Welsh,\u00a0C. (2018).\u00a0<em>Microbiology: A laboratory manual<\/em>.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>MacConkey Agar (Cappuccino &amp; Welsh, 2017). Description. MacConkey agar is selective and differential medium. It is selective for gram-negative bacteria as crystal violet (in MacConkey) inhibits gram-positive organisms. Lactose, bile salts, and pH indicator neutral red in MacConkey agar allow differentiation between coliform bacilli (lactose fermentation produces acidic end-products indicated by red-colored growth and pink [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[36],"tags":[],"class_list":["post-1133","post","type-post","status-publish","format-standard","hentry","category-microiology"],"_links":{"self":[{"href":"https:\/\/integrativewellnessandmovement.com\/iwmbasicscience\/wp-json\/wp\/v2\/posts\/1133","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/integrativewellnessandmovement.com\/iwmbasicscience\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/integrativewellnessandmovement.com\/iwmbasicscience\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/integrativewellnessandmovement.com\/iwmbasicscience\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/integrativewellnessandmovement.com\/iwmbasicscience\/wp-json\/wp\/v2\/comments?post=1133"}],"version-history":[{"count":1,"href":"https:\/\/integrativewellnessandmovement.com\/iwmbasicscience\/wp-json\/wp\/v2\/posts\/1133\/revisions"}],"predecessor-version":[{"id":1137,"href":"https:\/\/integrativewellnessandmovement.com\/iwmbasicscience\/wp-json\/wp\/v2\/posts\/1133\/revisions\/1137"}],"wp:attachment":[{"href":"https:\/\/integrativewellnessandmovement.com\/iwmbasicscience\/wp-json\/wp\/v2\/media?parent=1133"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/integrativewellnessandmovement.com\/iwmbasicscience\/wp-json\/wp\/v2\/categories?post=1133"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/integrativewellnessandmovement.com\/iwmbasicscience\/wp-json\/wp\/v2\/tags?post=1133"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}