{"id":1143,"date":"2018-08-14T02:38:32","date_gmt":"2018-08-14T02:38:32","guid":{"rendered":"https:\/\/integrativewellnessandmovement.com\/iwmbasicscience\/?p=1143"},"modified":"2018-08-14T02:50:11","modified_gmt":"2018-08-14T02:50:11","slug":"microbiology-hydrogensulfide","status":"publish","type":"post","link":"https:\/\/integrativewellnessandmovement.com\/iwmbasicscience\/microiology\/microbiology-hydrogensulfide\/","title":{"rendered":"Microbiology: Hydrogen sulfide test."},"content":{"rendered":"<p><strong>Hydrogen Sulfide Test <\/strong>(Cappuccino &amp; Welsh, 2017).<\/p>\n<p><strong>Description<\/strong>. Some bacteria are able to reduce organic\/inorganic sulfur sources (e.g. cysteine, thiosulfate) into hydrogen sulfide, H<sub>2<\/sub>S. The ferrous ammonium sulfate in the SIM agar (also containing peptone and sodium thiosulfate as substrates in the reduction\/hydrogenation of sulfur) deep medium acts as an indicator by forming black precipitate (positive result) in the presence of H<sub>2<\/sub>S gas (Cappuccino &amp; Welsh, 2017). The black precipitate &#8220;trail&#8221; also allows for observation of bacterial motility.<\/p>\n<p><strong>Clinical significance<\/strong>. The Hydrogen Sulfide test helps to separate <em>Proteus\/Salmonella<\/em> (able to metabolize sulfur) from <em>Shigella dysentariae<\/em> (unable to metabolize sulfur).<\/p>\n<p><strong>Summary of main steps<\/strong>. It is assumed that the reader knows aseptic technique and basic inoculation.<\/p>\n<ol>\n<li>Using aseptic technique and a transfer needle, inoculate a SIM agar deep tube via straight-line stab.<\/li>\n<li>Incubate tube at 37\u00b0C for 24-28 hours. Observe results.<\/li>\n<\/ol>\n<p><a href=\"https:\/\/integrativewellnessandmovement.com\/iwmbasicscience\/wp-content\/uploads\/2018\/08\/ShirleyChungHydrogenSulfideTest.jpg\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-1144\" src=\"https:\/\/integrativewellnessandmovement.com\/iwmbasicscience\/wp-content\/uploads\/2018\/08\/ShirleyChungHydrogenSulfideTest.jpg\" alt=\"\" width=\"864\" height=\"1274\" srcset=\"https:\/\/integrativewellnessandmovement.com\/iwmbasicscience\/wp-content\/uploads\/2018\/08\/ShirleyChungHydrogenSulfideTest.jpg 864w, https:\/\/integrativewellnessandmovement.com\/iwmbasicscience\/wp-content\/uploads\/2018\/08\/ShirleyChungHydrogenSulfideTest-203x300.jpg 203w, https:\/\/integrativewellnessandmovement.com\/iwmbasicscience\/wp-content\/uploads\/2018\/08\/ShirleyChungHydrogenSulfideTest-768x1132.jpg 768w, https:\/\/integrativewellnessandmovement.com\/iwmbasicscience\/wp-content\/uploads\/2018\/08\/ShirleyChungHydrogenSulfideTest-694x1024.jpg 694w\" sizes=\"auto, (max-width: 864px) 100vw, 864px\" \/><\/a><\/p>\n<p>Reference<\/p>\n<p>Cappuccino,\u00a0J.\u00a0G., &amp; Welsh,\u00a0C. (2018).\u00a0<em>Microbiology: A laboratory manual<\/em>.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Hydrogen Sulfide Test (Cappuccino &amp; Welsh, 2017). Description. Some bacteria are able to reduce organic\/inorganic sulfur sources (e.g. cysteine, thiosulfate) into hydrogen sulfide, H2S. The ferrous ammonium sulfate in the SIM agar (also containing peptone and sodium thiosulfate as substrates in the reduction\/hydrogenation of sulfur) deep medium acts as an indicator by forming black precipitate [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[36],"tags":[],"class_list":["post-1143","post","type-post","status-publish","format-standard","hentry","category-microiology"],"_links":{"self":[{"href":"https:\/\/integrativewellnessandmovement.com\/iwmbasicscience\/wp-json\/wp\/v2\/posts\/1143","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/integrativewellnessandmovement.com\/iwmbasicscience\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/integrativewellnessandmovement.com\/iwmbasicscience\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/integrativewellnessandmovement.com\/iwmbasicscience\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/integrativewellnessandmovement.com\/iwmbasicscience\/wp-json\/wp\/v2\/comments?post=1143"}],"version-history":[{"count":5,"href":"https:\/\/integrativewellnessandmovement.com\/iwmbasicscience\/wp-json\/wp\/v2\/posts\/1143\/revisions"}],"predecessor-version":[{"id":1154,"href":"https:\/\/integrativewellnessandmovement.com\/iwmbasicscience\/wp-json\/wp\/v2\/posts\/1143\/revisions\/1154"}],"wp:attachment":[{"href":"https:\/\/integrativewellnessandmovement.com\/iwmbasicscience\/wp-json\/wp\/v2\/media?parent=1143"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/integrativewellnessandmovement.com\/iwmbasicscience\/wp-json\/wp\/v2\/categories?post=1143"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/integrativewellnessandmovement.com\/iwmbasicscience\/wp-json\/wp\/v2\/tags?post=1143"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}