{"id":1172,"date":"2018-08-14T04:03:57","date_gmt":"2018-08-14T04:03:57","guid":{"rendered":"https:\/\/integrativewellnessandmovement.com\/iwmbasicscience\/?p=1172"},"modified":"2018-08-14T06:48:43","modified_gmt":"2018-08-14T06:48:43","slug":"microbiology-stainspart1","status":"publish","type":"post","link":"https:\/\/integrativewellnessandmovement.com\/iwmbasicscience\/microiology\/microbiology-stainspart1\/","title":{"rendered":"Microbiology: Stains, part 1."},"content":{"rendered":"<p>A stain is an organic compound composed of:<\/p>\n<ul>\n<li><strong>Benzene ring<\/strong>: organic colorless solvent.<\/li>\n<li><strong>Chromophore<\/strong>: the color component.<\/li>\n<li><strong>Chromogen<\/strong> = benzene component + chromophore component.<\/li>\n<li><strong>Auxochrome<\/strong>: helps to modify the chromophore&#8217;s ability to absorb light; and modifies the chromogen (ionization) to help it bind to tissue\/fibers.<\/li>\n<\/ul>\n<p><strong>Acidic stains<\/strong>: Sodium, potassium, calcium or ammonium salts ionize such that\u00a0chromogen has anionic properties and has affinity for the &#8220;positive&#8221; components of a cell (e.g. picric acid).<\/p>\n<p><strong>Basic stains<\/strong>: Chlorides or sulfate salts of colored bases ionize such that chromogen has cationic properties and has affinity for the &#8220;negative&#8221; components of a cell (methylene blue).<\/p>\n<p><strong>Differential stains<\/strong>: are more complex, use 2 contrasting stains, and help to separate organisms\/cells; and\/or help to visualize structures such as flagella, capsule, spore, or nucleus.<\/p>\n<p>Reference<\/p>\n<p>Cappuccino,\u00a0J.\u00a0G., &amp; Welsh,\u00a0C. (2018).\u00a0<em>Microbiology: A laboratory manual<\/em>.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>A stain is an organic compound composed of: Benzene ring: organic colorless solvent. Chromophore: the color component. Chromogen = benzene component + chromophore component. Auxochrome: helps to modify the chromophore&#8217;s ability to absorb light; and modifies the chromogen (ionization) to help it bind to tissue\/fibers. Acidic stains: Sodium, potassium, calcium or ammonium salts ionize such [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[36],"tags":[],"class_list":["post-1172","post","type-post","status-publish","format-standard","hentry","category-microiology"],"_links":{"self":[{"href":"https:\/\/integrativewellnessandmovement.com\/iwmbasicscience\/wp-json\/wp\/v2\/posts\/1172","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/integrativewellnessandmovement.com\/iwmbasicscience\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/integrativewellnessandmovement.com\/iwmbasicscience\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/integrativewellnessandmovement.com\/iwmbasicscience\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/integrativewellnessandmovement.com\/iwmbasicscience\/wp-json\/wp\/v2\/comments?post=1172"}],"version-history":[{"count":5,"href":"https:\/\/integrativewellnessandmovement.com\/iwmbasicscience\/wp-json\/wp\/v2\/posts\/1172\/revisions"}],"predecessor-version":[{"id":1183,"href":"https:\/\/integrativewellnessandmovement.com\/iwmbasicscience\/wp-json\/wp\/v2\/posts\/1172\/revisions\/1183"}],"wp:attachment":[{"href":"https:\/\/integrativewellnessandmovement.com\/iwmbasicscience\/wp-json\/wp\/v2\/media?parent=1172"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/integrativewellnessandmovement.com\/iwmbasicscience\/wp-json\/wp\/v2\/categories?post=1172"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/integrativewellnessandmovement.com\/iwmbasicscience\/wp-json\/wp\/v2\/tags?post=1172"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}