{"id":1180,"date":"2018-08-14T06:46:04","date_gmt":"2018-08-14T06:46:04","guid":{"rendered":"https:\/\/integrativewellnessandmovement.com\/iwmbasicscience\/?p=1180"},"modified":"2018-08-14T19:26:38","modified_gmt":"2018-08-14T19:26:38","slug":"microbiology-negative-stains-part3","status":"publish","type":"post","link":"https:\/\/integrativewellnessandmovement.com\/iwmbasicscience\/microiology\/microbiology-negative-stains-part3\/","title":{"rendered":"Microbiology: Negative stains, part 3."},"content":{"rendered":"<p><strong>Negative stains<\/strong>: uses an acidic stain like India ink or nigrosin. The anionic chromogen properties of the stain will not penetrate the cell\/organism thereby staining the &#8220;background&#8221; (negative space around the cell\/organism) instead. Heat fixing is not required so beware that the organisms\/bacteria are NOT killed. Aseptic technique and proper disposal is important.<\/p>\n<p><strong>Clinical significance<\/strong>. Helpful in detecting spirochetes (difficult to stain), presence of a capsule or spore. Helps identify infectious agents such as fungi from bird droppings.<\/p>\n<p><b>Summary of procedure.<\/b><\/p>\n<ol>\n<li>Place a drop of India ink or nigrosin on one end of a clean slide.<\/li>\n<li>Use a loopful of inoculated broth and mix it well with the drop of stain on the slide.<\/li>\n<li>Place the short edge of another slide at a 45 degree slant against the spot of stain. Drag this second slide across the original slide (smearing the drop across).<\/li>\n<\/ol>\n<p>Reference<\/p>\n<p>Cappuccino,\u00a0J.\u00a0G., &amp; Welsh,\u00a0C. (2018).\u00a0<em>Microbiology: A laboratory manual<\/em>.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Negative stains: uses an acidic stain like India ink or nigrosin. The anionic chromogen properties of the stain will not penetrate the cell\/organism thereby staining the &#8220;background&#8221; (negative space around the cell\/organism) instead. Heat fixing is not required so beware that the organisms\/bacteria are NOT killed. Aseptic technique and proper disposal is important. Clinical significance. [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[36],"tags":[],"class_list":["post-1180","post","type-post","status-publish","format-standard","hentry","category-microiology"],"_links":{"self":[{"href":"https:\/\/integrativewellnessandmovement.com\/iwmbasicscience\/wp-json\/wp\/v2\/posts\/1180","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/integrativewellnessandmovement.com\/iwmbasicscience\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/integrativewellnessandmovement.com\/iwmbasicscience\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/integrativewellnessandmovement.com\/iwmbasicscience\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/integrativewellnessandmovement.com\/iwmbasicscience\/wp-json\/wp\/v2\/comments?post=1180"}],"version-history":[{"count":1,"href":"https:\/\/integrativewellnessandmovement.com\/iwmbasicscience\/wp-json\/wp\/v2\/posts\/1180\/revisions"}],"predecessor-version":[{"id":1181,"href":"https:\/\/integrativewellnessandmovement.com\/iwmbasicscience\/wp-json\/wp\/v2\/posts\/1180\/revisions\/1181"}],"wp:attachment":[{"href":"https:\/\/integrativewellnessandmovement.com\/iwmbasicscience\/wp-json\/wp\/v2\/media?parent=1180"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/integrativewellnessandmovement.com\/iwmbasicscience\/wp-json\/wp\/v2\/categories?post=1180"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/integrativewellnessandmovement.com\/iwmbasicscience\/wp-json\/wp\/v2\/tags?post=1180"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}