{"id":1206,"date":"2018-08-14T20:34:16","date_gmt":"2018-08-14T20:34:16","guid":{"rendered":"https:\/\/integrativewellnessandmovement.com\/iwmbasicscience\/?p=1206"},"modified":"2018-08-14T20:34:48","modified_gmt":"2018-08-14T20:34:48","slug":"microbiology-capsule-staining-part-7","status":"publish","type":"post","link":"https:\/\/integrativewellnessandmovement.com\/iwmbasicscience\/microiology\/microbiology-capsule-staining-part-7\/","title":{"rendered":"Microbiology: Capsule staining, part 7."},"content":{"rendered":"<p><strong>Capsule staining (Anthony method)<\/strong>: is atypical and cells that have a gelatinous capsule (complex polysaccharides, levans, dextrans, cellulose) are often by nature virulent. Capsule staining is difficult because the capsule is water-soluble\u2014easy to washout stain or destroy the capsule. There are 2 reagents.<\/p>\n<p>The <strong>primary stain<\/strong> is crystal violet (stains cell and capsule).<\/p>\n<p>The <strong>decolorizing agent<\/strong> is copper sulfate (20%) which takes out the primary stain from the capsule but does not remove color bound to the cell wall. Copper sulfate will also bind to the capsular material.<\/p>\n<p><strong>Clinical significance<\/strong>. Helps to identify capsule-forming Gram-negative bacteria such as <em>Haemophilus influenzae<\/em> and <em>Klebsiella pneumoniae<\/em>. Capsule-forming Gram-positive bacteria include <em>Bacillus anthracis<\/em> and <em>Streptococcus pneumoniae<\/em>.<\/p>\n<p><b>Summary of procedure.<\/b><\/p>\n<ol>\n<li>On a clean slide, put several drops of crystal violet.<\/li>\n<li>Inoculate the slide (with crystal violet) with 3 loopfuls of a culture.<\/li>\n<li>Using another clean slide, smear the first slide such that the inoculated crystal violet spreads thinly across.<\/li>\n<li>Allow this to stand for 5-7 minutes such that it is dry.<\/li>\n<li>Do not heatfix.<\/li>\n<li>Gently flush smear with copper sulfate solution.<\/li>\n<li>Gently blot with bibulous paper.<\/li>\n<\/ol>\n<p>Reference<\/p>\n<p>Cappuccino,\u00a0J.\u00a0G., &amp; Welsh,\u00a0C. (2018).\u00a0<em>Microbiology: A laboratory manual<\/em>.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Capsule staining (Anthony method): is atypical and cells that have a gelatinous capsule (complex polysaccharides, levans, dextrans, cellulose) are often by nature virulent. Capsule staining is difficult because the capsule is water-soluble\u2014easy to washout stain or destroy the capsule. There are 2 reagents. The primary stain is crystal violet (stains cell and capsule). The decolorizing [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[36],"tags":[],"class_list":["post-1206","post","type-post","status-publish","format-standard","hentry","category-microiology"],"_links":{"self":[{"href":"https:\/\/integrativewellnessandmovement.com\/iwmbasicscience\/wp-json\/wp\/v2\/posts\/1206","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/integrativewellnessandmovement.com\/iwmbasicscience\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/integrativewellnessandmovement.com\/iwmbasicscience\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/integrativewellnessandmovement.com\/iwmbasicscience\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/integrativewellnessandmovement.com\/iwmbasicscience\/wp-json\/wp\/v2\/comments?post=1206"}],"version-history":[{"count":2,"href":"https:\/\/integrativewellnessandmovement.com\/iwmbasicscience\/wp-json\/wp\/v2\/posts\/1206\/revisions"}],"predecessor-version":[{"id":1208,"href":"https:\/\/integrativewellnessandmovement.com\/iwmbasicscience\/wp-json\/wp\/v2\/posts\/1206\/revisions\/1208"}],"wp:attachment":[{"href":"https:\/\/integrativewellnessandmovement.com\/iwmbasicscience\/wp-json\/wp\/v2\/media?parent=1206"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/integrativewellnessandmovement.com\/iwmbasicscience\/wp-json\/wp\/v2\/categories?post=1206"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/integrativewellnessandmovement.com\/iwmbasicscience\/wp-json\/wp\/v2\/tags?post=1206"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}