Microbiology: Broth tube.

Inoculations

Broth tube (Cappuccino & Welsh, 2017). Using aseptic technique:

1. Flame loop. Flame opening of broth culture.
2. Dip loop into broth culture. Flame opening of broth culture.
3. Open the sterile new broth tube. Flame opening of the sterile broth tube.
4. Dip loop into the sterile broth and gently shake the loop inside the new broth.

Flame the opening of the new broth tube and replace cap. Flame loop.

Reference

Cappuccino, J. G., & Welsh, C. (2018). Microbiology: A laboratory manual.