Microbiology Lab Media/Test Notes
Microbiology Lab Notes (media and tests) By ©2018 Shirley S. Chung, Green River Community College Download these notes. Thioglycollate Broth: ...
Microbiology Lab Procedure Notes
Microbiology Lab Procedure Notes By ©2018 Shirley S. Chung, Green River Community College Download these notes. Smear Clean slide Label: ...
Microbiology: Acid-Fast stains, part 5.
Acid-Fast stains: are particularly helpful when the organism's cell wall is thick and waxy (lipoidal)—resisting the other (more conventional) staining ...
Microbiology: Anaerobic GasPak system.
Anaerobic GasPak System (Cappuccino & Welsh, 2017). Description. The GasPak is a sealed jar system that produces an anaerobic (non-reducing) ...
Microbiology: Bacterial growth phases.
The general bacterial growth curve has 4 phases: lag, logarithmic (log), stationary, and decline/death. You can remember this by "LLSD" ...
Microbiology: Basic media/medium for growing cultures.
In order to grow organisms, you need to provide an adequate environment suitable for their growth. Things to consider are ...
Microbiology: Biochemicals and Enzymes.
Microorganisms may be described and characterized by their biochemical/metabolic functions, including their use of enzymes. Here are some terms to ...
Microbiology: Broth tube.
Inoculations Broth tube (Cappuccino & Welsh, 2017). Using aseptic technique: Flame loop. Flame opening of broth culture. Dip loop into ...
Microbiology: Capsule staining, part 7.
Capsule staining (Anthony method): is atypical and cells that have a gelatinous capsule (complex polysaccharides, levans, dextrans, cellulose) are often ...
Microbiology: Carbohydrate fermentation tests.
Carbohydrate Fermentation Tests (Cappuccino & Welsh, 2017). Description. Different bacteria have different carbohydrate fermenting profiles. The series of three carbohydrate ...
Microbiology: Carbohydrate Fermentation.
Carbohydrates may be metabolized in many different ways: aerobically, anaerobically, both or neither. In carbohydrate (or alcohols) fermentation, anaerobic dissimilation ...
Microbiology: Catalase test.
Aerobes, facultative anaerobes, and microaerophiles may produce hydrogen peroxide and/or superoxide as a result of aerobic respiration (oxygen is final ...
Microbiology: Citrate test.
Citrate Test (Cappuccino & Welsh, 2017). Description. Simmons citrate agar containing pH bromthymol blue indicator changes from green (prior to ...
Microbiology: Dichotomous Key
Designing the Dichotomous Key When designing a dichotomous key, each level represents 1 step in your logic/questioning. The "di" in ...
Microbiology: Differential stains, part 4.
Differential stains: are more complex, using 4 reagents (the order is important), and help to separate organisms/cells; and/or help to ...
Microbiology: Effects of pH.
The environment's pH affects the growth of organisms. Generally, bacteria grows between pH 4-9 and optimally between pH 6.5-7.5. Fungi ...
Microbiology: Effects of temperature.
Different organisms grow best at different temperature ranges. Temperature ranges (cardinal temperature points) may be described by three points (the ...
Microbiology: Extracellular Enzymes.
Large and/or complex molecules/substances/nutrients (e.g. polysaccharides, lipids, proteins) need to be broken down into simpler parts before they are able ...
Microbiology: Getting started talking about cultures.
Let's get started talking about cultures. Pure culture: a culture that contains or is made up of a single species ...
Microbiology: Gram stain.
Gram Staining (Cappuccino & Welsh, 2017). Description. Gram staining is a differential staining technique that differentiates bacteria based on their ...
