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Microbiology: Slant tube.

Inoculations

Agar slant (Cappuccino & Welsh, 2017). Using aseptic technique:

  1. Flame loop. Flame opening of broth culture.
  2. Dip loop into broth culture. Flame opening of broth culture.
  3. Open the sterile slant. Flame opening of the sterile slant.
  4. Using the loop (which has been dipped into the broth culture), drag loop across the agar slant in a zig-zag pattern from the end (bottom) up to the opening.
  5. Flame opening of slant tube and replace the cap. Flame loop.

Reference

Cappuccino, J. G., & Welsh, C. (2018). Microbiology: A laboratory manual.